Preparation procedure and effects of elisa kit experiments
Preparation procedure of ELISA kit:
1. Acidified urine or tissue homogenate increased the pH of 2 million hydrochloric acid by 3.5. Allow to sit at 4° for 15
minute. The precipitate was removed in the centrifuge sample for 2 minutes.
2. Prepare a C18 reverse phase column to wash 10 ml of ethanol followed by 10 ml of deionized water.
3. The sample was subjected to a slight positive pressure to obtain a flow rate of about 0.5 ml/min. The ELISA kit was washed with 10 ml of water, followed by 10 ml of 15% ethanol and finally 10 ml of hexane. Add 10 ml of ethyl acetate to the elution sample column.
4. If the analysis is performed immediately, the nitrogen flow under the sample is evaporated. Add at least a dry sample of 250 μ credit analysis buffer. The vortex then allows to sit for 5 minutes in the room temperature. Repeat twice. If the ELISA kit analysis is delayed, e.g., elute the sample solution at -80° until the immunization is run. The nitrogen under the evaporation of the organic solvent stream was tested and recombined as above before running.
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